REVIEWS
2015 Jorgenson LA et al. 2015  The BRAIN Initiative: developing technology to catalyse neuroscience discovery. Phil. Trans. R. Soc. B 370
2015 Kevin L. Briggman, William B. Kristan, Jesus E. Gonzalez, David Kleinfeld, and Roger Y. Tsien.  Chapter 6 - Monitoring Integrated Activity of Individual Neurons Using FRET-Based Voltage-Sensitive Dyes.   Springer International Publishing Switzerland.  Membrane Potential Imaging in the Nervous System and Heart, Advances in Experimental Medicine and Biology,  149-169
2015 B. Albert Griffin , Stephen R. Adams , and Roger Y. Tsien.   Chapter 1 - How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag.  Springer Science Site-Specific Protein Labeling: Methods and Protocols, Methods in Molecular Biology, vol. 1266: 1-61
2015 Adamantidis et al.  Optogenetics: 10 years after ChR2 in neurons—views from the community.  Nature Neuroscience 18:9 1202-1212
2013 Roger Y. Tsien  2013. Very long-term memories may be stored in the pattern of holes in the perineuronal net.  PNAS 110:30
2010 Roger Y. Tsien  2010. Fluorescence readouts of biochemistry in live cells and organisms. In Molecular Imaging: Principles and Practic ed. by R. Weissleder, S.S. Gambhir, B.D. Ross, A. Rehemtulla. People’s Medical Publishing House – USA. Chapter 48, pp. 808-828
2009 Roger Y. Tsien  2009. Constructing and exploiting the fluorescent protein paintbox (Autobiography + Nobel Lecture), in Les Prix Nobel 2008, pp. 171-214. Nobel Lecture reprinted (2009) in Angewandte Chemie Intl. Edition 48: 5612-5626.
2009 Roger Y. Tsien 2009  Constructing and Exploiting the Fluorescent Protein Paintbox (Nobel Lecture). Angew. Chem. Int. Ed.  48: 5612 – 5626
2006 Wang, L., and Tsien, R.Y. 2006. Evolving proteins in mammalian cells using somatic hypermutation. Nature Protocols 1: 1346-1350.
2006 Stephen R. Adams & Roger Y. Tsien  2006 Imaging the influx of cell-penetrating peptides into the cytosol of individual cells. Chapter 29 in: Handbook of Cell-Penetrating Peptides, ed by Ulo Langel (2nd edition). CRC 505-512.
2006 Amy E. Palmer and Roger Y. Tsien 2006  Measuring calcium signaling using genetically targetable fluorescent indicators.  Nature Protocols, Vol 1: 1057-1065.
2006 Ben N. G. Giepmans, Stephen R. Adams,  Mark H. Ellisman & Roger Y. Tsien 2006 The fluorescent toolbox for assessing protein location and function. Science. 312: 217-224.
2006 Zacharias, D.A., and Tsien, R.Y.  Molecular biology and mutation of green fluorescent protein.  Chapter 5 in: Green Fluorescent Protein: Properties, Applications, and Protocols, ed. by M. Chalfie and S.R. Kain (2nd edition). John Wiley & Sons. This article is listed in Medline as Methods Biochem. Anal. 2006; 47: 83-120
2005 Tsien, R.Y. 2005. Breeding molecules to spy on cells, in The Harvey Lectures: Series 99, 2003-2004. Harvey Society, 2005
2005

Nathan C. Shaner, Paul A. Steinbach, & Roger Y. Tsien. 2005 . A guide to choosing fluorescent proteins, Nature Methods, Vol. 2: 905 – 909
2003

Tsien, R.Y. 2003 Imagining imaging's future. Nature Reviews Molecular Cell Biology 4: SS16-SS21
2002

Zhang, J., Campbell, R.E., Ting, A. and Tsien, R.Y. 2002.  Creating new fluorescent probes for cell biology. Nature Review Molecular Biology 3: 906-918.
2000

Miyawaki, A., and Tsien, R.Y. 2000. Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein. Methods in Enzymology 327: 472-500.
2000 Wu, M.M., Llopis, J., Adams, S., McCaffery, J.M., Teter, K., Kulomaa, M.S., Machen, T.E.,Moore,H.-P.H., and Tsien, R.Y. 2000  Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates.  Methods in Enzymology.327: 546-564.
2000

Griffin, B.A., Adams, S.R., Jones, J., and Tsien, R.Y. 2000. Fluorescent labeling of recombinant proteins in living cells with FlAsH 2001. Methods in Enzymology. 327: 565-578.
2000 Zacharias, D.A., Baird, G.S., and Tsien, R.Y. 2000.  Recent advances in technology for measuring and manipulating cell signals. Current Opinion in Neurobiology 10: 416-421.
1999 Tsien, R.Y. 1999. Monitoring Cell Calcium.  In Calcium as a Cellular Regulator, ed. by E. Carafoli and C. Klee. New York: Oxford University Press, pp. 28-54.
1998 Tsien, R.Y. 1998. The Green Fluorescent Protein. Annu. Rev. Biochemistry 67: 509-544.
1989 Tsien. R.Y. and Pozzan T. 1989. Measurement of Cytosolic Free Ca2+ with Quin2.  Method in Enzymology. 172:  230-244.

 

PAPERS
2016 Stephen R. Adams, Mason R. Mackey, Ranjan Ramachandra, Sakina F. Palida Lemieux, Paul Steinbach, Eric A. Bushong, Margaret T. Butko, Ben N.G. Giepmans, Mark H. Ellisman, and Roger Y. Tsien.  Multicolor Electron Microscopy for Simultaneous Visualization of Multiple Molecular Species. Cell Chemical Biology 23, 1417–1427
2016 Erik A. Rodriguez, Geraldine N. Tran, Larry A. Gross, Jessica C. Crisp, Xiaokun Shu, John Y. Lin, & Roger Y. Tsien. A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein. Nature Methods 13: 763-769               Supplemental
2016 Alexander A. Kislukhin, Hongyan Xu, Stephen R. Adams, Kazim H. Narsinh, Roger Y. Tsien and Eric T. Ahrens  Paramagnetic fluorinated nanoemulsions for sensitive cellular fluorine-19 magnetic resonance imaging. Nature Materials 15: 662-668
2016 John T Ngo1, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark HEllisman & Roger Y Tsien  Click-EM for imaging metabolically tagged nonprotein biomolecules.  Nataure Chemical Biology 12: 459-468
2016 Ryan K. Orosco, MD, Elamprakash N. Savariar, PhD, Philip A. Weissbrod, MD, Julio A. DiazPerez, MD, Michael Bouvet, MD, Roger Y. Tsien, PhD, & Quyen T. Nguyen  Molecular Targeting of Papillary Thyroid Carcinoma With Fluorescently Labeled Ratiometric Activatable Cell Penetrating Peptides in a Transgenic Murine Model.  J of Surgical Oncology 113: 138–143
2016 Erik A. Rodriguez, Ye Wang, Jessica L. Crisp, David R. Vera, Roger Y. Tsien and Richard Ting  New Dioxaborolane Chemistry Enables [18F]-Positron-Emitting, Fluorescent [18F]-Multimodality Biomolecule Generation from the Solid Phase.  Bioconjugate Chemistry  27: 1390-1399
2015 Ning Hua, Fred Baik, Tuan Pham, Alkystis Phinikaridou, Nick Giordano, Beth Friedman, Michael Whitney, Quyen T. Nguyen, Roger Y. Tsien, James A.
Hamilton.  Identification of High-Risk Plaques by MRI and Fluorescence Imaging in a Rabbit Model of Atherothrombosis. PLoS ONE 10 1-15
2015 Shanyan Chen, Jiankun Cui1, Tao Jiang, Emilia S Olson, Quan-Yu Cai, Ming Yang, WeiWu, James M Guthrie, JD Robertson, Stuart A Lipton, Lixin Ma,
Roger Y Tsien and Zezong Gu.  Gelatinase activity imaged by activatable cell-penetrating peptides in cell-based and in vivo models of stroke. Journal of Cerebral Blood Flow & Metabolism 0 1-13
2015 Christopher D. Malone, Emilia S. Olson, Robert F. Mattrey, Tao Jiang, Roger Y. Tsien, Quyen T. Nguyen.  Tumor Detection at 3 Tesla with an Activatable Cell Penetrating Peptide Dendrimer (ACPPD-Gd), a T1 Magnetic Resonance (MR) Molecular Imaging Agent. PLoS ONE 9:3 1-15
2015 Lisa Buckel, Elamprakash N. Savariar, Jessica L. Crisp, Karra A. Jones, Angel M. Hicks, Daniel J. Scanderbeg, Quyen T. Nguyen, Jason K. Sicklick, Andrew M. Lowy, Roger Y. Tsien, and Sunil J. Advani. Tumor Radiosensitization by Monomethyl Auristatin E: Mechanism of Action and Targeted Delivery. Cancer Research 75:7 1376-1387
2015 Timon Hussain, Melina B. Mastrodimos, Sharat C. Raju, Heather L. Glasgow, Michael Whitney, Beth Friedman, Jeffrey D. Moore, David Kleinfeld, Paul Steinbach, Karen Messer, Minya Pu, Roger Y. Tsien, Quyen T. Nguyen.  Fluorescently Labeled Peptide Increases Identification of Degenerated Facial Nerve Branches during Surgery and Improves Functional Outcome.  PLoS ONE 3:9 1-13
2015 Hokyung K Chung, Conor L Jacobs, Yunwen Huo, Jin Yang, Stefanie A Krumm, Richard K Plemper, Roger Y Tsien & Michael Z Lin.  Tunable and reversible drug control of protein production via a self-excising degron.  Nature Chem Bio 11 713-722
2015 Sharat C. Raju, Samantha J. Hauff, Aaron J. Lemieux, Ryan K. Orosco, Andrew M. Gross, Linda T. Nguyen, Elamprakash Savariar, William Moss, Michael Whitney, Ezra E. Cohen, Scott M. Lippman, Roger Y. Tsien, Trey Ideker, Sunil J. Advani, Quyen T. Nguyen.  Combined TP53 mutation/3p loss correlates with decreased radiosensitivity and increased matrix-metalloproteinase activity in head and neck carcinoma.  Oral Oncology 51 470–475
2015 Clifford R. Woodford, E. Paxon Frady, Richard S. Smith, Benjamin Morey,Gabriele Canzi, Sakina F. Palida, Ricardo C. Araneda, William B. Kristan, Jr., Clifford P. Kubiak Evan W. Miller, and Roger Y. Tsien.  Improved PeT Molecules for Optically Sensing Voltage in Neurons.  J. Am. Chem. Soc. 137 1817−1824
2015 Sakina F. Palida, Margaret T. Butko, John T. Ngo, Mason R. Mackey, Larry A. Gross, Mark H. Ellisman, and Roger Y. Tsien.  PKM But Not PKC Is Rapidly Synthesized and Degraded at the Neuronal Synapse.  J. Neuroscience   35(20): 7736 –7749
2014 Timon Hussain, MD; Elamprakash N. Savariar; Julio A. Diaz Perez, MD; Karen Messer; Minya Pu; Roger Y. Tsien,; Quyen T. Nguyen, MD.  Surgical molecular navigation with a Ratiometric Activatable Cell Penetrating Peptide improves intraoperative identification and resection of small salivary gland cancers.  Accepted Article
2014 Samantha J. Hauff, Sharat C. Raju, Ryan K. Orosco, Andrew M. Gross, Julio A. Diaz-Perez, Elamprakash Savariar,Nadia Nashi, Jonathan Hasselman, Michael Whitney, Jeffrey N. Myers, Scott M. Lippman, Roger Y. Tsien, Trey Ideker and Quyen T. Nguyen.  Matrix-Metalloproteinases in Head and Neck Carcinoma-Cancer Genome Atlas Analysis and Fluorescence Imaging in Mice.  Otolaryngology -- Head and Neck Surgery 151: 612-618
2014 Cristina A. Metildi, MD, Csilla N. Felsen, Elamprakash N. Savariar, Quyen T. Nguyen, MD, Sharmeela Kaushal, Robert M. Hoffman, Roger Y. Tsien, and Michael Bouvet, MD. 
Ratiometric Activatable Cell-Penetrating Peptides Label Pancreatic Cancer, EnablingFluorescence Guided Surgery, Which Reduces Metastases and Recurrence in Orthotopic Mouse Models. Ann Surg Oncol
2014 Sadegh Nabavi, Rocky Fox, Christophe D. Proulx, John Y. Lin, Roger Y. Tsien & Roberto Malinow.  Engineering a memory with LTD and LTP.  Nature. 511
2014 Rachel A. Levin, Csilla N. Felsen, Jin Yang, John Y. Lin, Michael A. Whitney, Quyen T. Nguyen, Roger Y. Tsien.  An Optimized Triple Modality Reporter for Quantitative In Vivo Tumor Imaging and Therapy Evaluation.  www.plosone.org 9:5 1-10
2014 Jessica L. Crisp, Elamprakash N. Savariar, Heather L. Glasgow, Lesley G.Ellies, Michael A. Whitney, Roger. Y. Tsien. Dual targeting of integrin alphavbeta3 and matrix metalloproteinase-2 for optical imaging of tumors and chemotherapeutic delivery.  Molelcular Cancer Therapeutics.
2014 Dimitrios Davalos, Kim M. Baeten, Michael A. Whitney, Eric S. Mullins, Beth Friedman, Emilia S. Olson, Jae Kyu Ryu, Dimitri S. Smirnoff, Mark A. Petersen, Catherine Bedard, Jay L. Degen, Roger Y. Tsien, and Katerina Akassoglou. Early Detection of Thrombin Activity in Neuroinflammatory Disease. Annals of Neurology 75:303–308
2014 Inanc Ortac, Dmitri Simberg, Ya-san Yeh, Jian Yang, Bradley Messmer, William C. Trogler, Roger Y. Tsien, and Sadik Esener.  Dual-Porosity Hollow Nanoparticles for the Immunoprotection and Delivery of Nonhuman Enzymes.  Nano Letters
2014 Hidehiko K Inagaki1, Yonil Jung1, Eric D Hoopfer, Allan M Wong, Neeli Mishra, John Y Lin, Roger Y Tsien & David J Anderson.  Optogenetic control of Drosophila using a red-shifted channelrhodopsin reveals experience-dependent influences on courtship.  Nature Methods
2014 Csilla N. Felsen, Elamprakash N. Savariar, Michael Whitney, and Roger Y. Tsien.  Detection and monitoring of localized matrix metalloproteinase upregulation in a murine model of asthma.  Am J Physiol Lung Cell Mol Physiol  306: L764–L774
2014 Roy Weinstain, Elamprakash N. Savariar, Csilla N. Felsen, and Roger Y. Tsien. In Vivo Targeting of Hydrogen Peroxide by Activatable Cell-Penetrating Peptides. J. Am. Chem. Soc. 136:874-877
2013 Roy Weinstain, Joan Kanter, Beth Friedman, Lesley G. Ellies, Michael E. Baker,and Roger Y. Tsien. Fluorescent Ligand for Human Progesterone Receptor Imaging in Live Cells. Bioconjugate Chem. 24:766-771
2013 John Y Lin, Per Magne Knutsen, Arnaud Muller, David Kleinfeld & Roger Y Tsien.  ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation.  Nature Neuroscience 16:10 1499-1508
2013 John Y. Lin, Sharon B.Sann, Keming Zhou, Sadegh Nabavi, Christophe D. Proulx, Roberto Malinow, Yishi Jin and Roger Y. Tsien.  Optogenetic Inhibition of Synaptic Release with Chromophore-Assisted Light Inactivation (CALI).  Neuron 79: 241–253
2013 Michael Whitney, Elamprakash N. Savariar, Beth Friedman, Rachel A. Levin, Jessica L. Crisp, Heather L. Glasgow, Roy Lefkowitz, Stephen R. Adams, Paul Steinbach, Nadia Nashi, Quyen T. Nguyen, and Roger Y. Tsien 2013.  Ratiometric Activatable Cell-Penetrating Peptides Provide Rapid In Vivo Readout of Thrombin Activation.  Angew. Chem. Int. 52 325–330
2013 Margaret T. Butko, Jeffrey N. Savas, Beth Friedman, Claire Delahunty, Ford Ebner, John R. Yates III, and Roger Y. Tsien 2013.  In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.  PNAS
2013 Eilon Shania, Roy Weinstain, Yi Zhang, Cristina Castillejo, Eirini Kaiserli, Joanne Chory, Roger Y. Tsien, and Mark Estelle 2013.  Gibberellins accumulate in the elongating endodermal cells of Arabidopsis root.  PNAS
2013 Elamprakash N. Savariar, Csilla N. Felsen, Nadia Nashi, Tao Jiang, Lesley G. Ellies, Paul Steinbach, Roger Y. Tsien, and Quyen T. Nguyen 2013.  Real-time In Vivo Molecular Detection of Primary Tumors and Metastases with Ratiometric Activatable Cell-Penetrating Peptides.  Cancer Res 73:2 855-864
2012 Margaret T Butko, Jin Yang, Yang Geng, Hyung Joon Kim, Noo Li Jeon, Xiaokun Shu, Mason R Mackey, Mark H Ellisman, Roger Y Tsien & Michael Z Lin 2012.  Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy.  Nature Neuroscience. 15:12 1742-1751
2012 Horng D. Ou, Witek Kwiatkowski, Thomas J. Deerinck, Andrew Noske, Katie Y. Blain, Hannah S. Land, Conrado Soria, Colin J. Powers, Andrew P. May, Xiaokun Shu,  Roger Y. Tsien, James A.J. Fitzpatrick, Jeff A. Long, Mark H. Ellisman, Senyon Choe, and Clodagh C. O’Shea 2012.  A Structural Basis for the Assembly and Functions of a Viral Polymer that Inactivates Multiple Tumor Suppressors.  Cell 151: 304–319
2012 Amy J Lam, François St-Pierre, Yiyang Gong, Jesse D Marshall, Paula J Cranfill, Michelle A Baird, Michael R McKeown, Jörg Wiedenmann, Michael W Davidson, Mark J Schnitzer, Roger Y Tsien, & Michael Z Lin 2012.  Improving FRET dynamic range with bright green and red fluorescent proteins.  Nature Methods 9:10  1005-1012
2012 Yingchuan B. Qi, Emma J. Garren, Xiaokun Shu, Roger Y. Tsien, and Yishi Jin 2012.  Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG.  PNAS 109 :19  7499–7504
2012 Emilia S. Olson, Michael A. Whitney, Beth Friedman, Todd A. Aguilera, Jessica L. Crisp, Fred M. Baik, Tao Jiang, Stephen M. Baird, Sotirios Tsimikas, Roger Y. Tsien and Quyen T. Nguyen 2012.  In vivo fluorescence imaging of atherosclerotic plaques with activatable cell-penetrating peptides targeting thrombin activity.  Integr. Biol.,  4: 595–605
2012 Xiaokun Shu1, Varda Lev-Ram, Thomas J. Deerinck, Yingchuan Qi1, Ericka B. Ramko, Michael W. Davidson, Yishi Jin1, Mark H. Ellisman, Roger Y. Tsien 2012.  A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms.  PLoS Biology,  9:4
2012 Yingchuan B. Qia, Emma J. Garrena, Xiaokun Shub, Roger Y. Tsien, and Yishi Jina 2012.  Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG.  PNAS 109: 7499–7504
2012 Evan W. Miller, John Y. Lina, E. Paxon Frady, Paul A. Steinbach, William B. Kristan, Jr., and Roger Y. Tsien 2012.  Optically monitoring voltage in neurons by photoinduced electron transfer through molecular wires.  PNAS 109: 2114–2119
2012 Bo Chen, Beth Friedman, Michael A. Whitney, Jessica A. Van Winkle, I-Farn Lei, Emilia S. Olson, Qun Cheng, Benedict Pereira,Lifu Zhao, Roger Y. Tsien, and Patrick D. Lyden 2012. Thrombin Activity Associated with Neuronal Damage during Acute Focal Ischemia, J. Neuro Science,  32:7622–7631
2011 Haiyan Li , Sarah M.Foss, Yuriy L.Dobryy, C. Kevin Park, Samuel Andrew Hires, Nathan C.Shaner, Roger Y. Tsien, Leslie C. Osborne and Susan M.Voglmaier 2011.  Concurrent imaging of synaptic vesicle recycling and calcium dynamics.  Frontiers in Molecular Neuroscience, 4: Article 34
2011 Xiaokun Shu, Varda Lev-Ram, Thomas J. Deerinck, Yingchuan Qi, Ericka B. Ramko, Michael W. Davidson, Yishi Jin, Mark H. Ellisman, Roger Y. Tsien 2011. A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms. Plosbiology, 9:1-10
2011 Michael A Whitney, Jessica L Crisp, Linda T Nguyen, Beth Friedman, Larry A Gross, Paul Steinbach, Roger Y Tsien & Quyen T Nguyen 2011.  Fluorescent peptides highlight peripheral nerves during surgery in mice. Nature Biotechnol. 2011; 29:352-6.
2011 Amy P. Wu, Michael A. Whitney, Jessica L. Crisp, Beth Friedman, Roger Y. Tsien, Quyen T. Nguyen 2011.  Improved Facial Nerve Identification with Novel Fluorescently Labeled Probe.  Laryngoscope. 121:805-10.
2010 Richard Ting, Todd A. Aguilera, Jessica L. Crisp, David J. Hall, William C. Eckelman, David R. Vera, and Roger Y. Tsien 2010.  Fast 18F Labeling of a Near-Infrared Fluorophore Enables Positron Emission Tomography and Optical Imaging of Sentinel Lymph Nodes. Bioconjugate Chem. 21:1811-1819
2010 Ouyang, M., Huang, H., Shaner, N.C., Remacle, A.G., Shiryaev, S.A., Strongin, A.Y., Tsien, R.Y., and Wang, Y. 2010. Simultaneous visualization of protumorigenic Src and MT1-MMP activities with fluorescence resonance energy transfer. Cancer Research 70: 2204-2212
2010 Whitney, M., Crisp, J.L., Olson, E.S., Aguilera, T.A., Gross, L.A., Ellies, L.G., and Tsien, R.Y. 2010. Parallel in vivo and in vitro selection using phage display identifies protease dependent tumor targeting peptides. J. Biol. Chem. 285: 22532-22541
2010

Emilia S. Olson, Tao Jiang, Todd A. Aguilera, Quyen T. Nguyen, Lesley G. Ellies, Miriam Scadeng, and Roger Y. Tsien 2010  Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases.     Proc. Natl. Acad. Sci. 107 : 4311–4316

 

Supplemental
2010

Quyen T. Nguyen, Emilia S. Olson, Todd A. Aguilera, Tao Jiang, Miriam Scadeng, Lesley G. Ellies,and Roger Y. Tsien 2010  Surgery with molecular fluorescence imaging using activatable cell-penetrating peptides decreases residual cancer and improves survival.    Proc. Natl. Acad. Sci. 107 : 4317–4322

 

Supplemental
2009 Graham T. Dempsey, Mark Bates, Walter E. Kowtoniuk, David R. Liu, Roger Y. Tsien, and Xiaowei Zhuang 2009  Photoswitching Mechanism of Cyanine Dyes.  J Am. Chem. Soc. 131: 18192–18193
2009 Michael Z. Lin, Michael R. McKeown, Ho-Leung Ng, Todd A. Aguilera, Nathan C. Shaner, Robert E. Campbell, Stephen R. Adams, Larry A. Gross, Wendy Ma, Tom Alber, and Roger Y. Tsien 2009  Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals. Chemistry & Biology 16: 1169–1179
2009

Todd A. Aguilera, Emilia S. Olson, Margaret M. Timmers, Tao Jiang and Roger Y. Tsien 2009  Systemic in vivo distribution of activatable cell penetrating peptides is superior to that of cell penetrating peptides.  Integr. Biol. 1: 371–381

 

Supplemental
2009

Emilia S. Olson, Todd A. Aguilera, Tao Jiang, Lesley G. Ellies, Quyen T. Nguyen, Edmund H. Wong, Larry A. Gross and Roger Y. Tsien 2009  In vivo characterization of activatable cell penetrating peptides for targeting protease activity in cancer.  Integr. Biol. 1: 382–393

 

Supplemental
2009

Fatemeh Madani, Jesper Lind, Peter Damberg, Stephen R. Adams, Roger Y. Tsien, and Astrid O. Graslund 2009  Hairpin Structure of a Biarsenical-Tetracysteine Motif Determined by NMR Spectroscopy.  J. AM. CHEM. SOC. 131: 4613–4615

 

Supplemental
2009

Xiaokun Shu, Antoine Royant, Michael Z. Lin, Todd A. Aguilera, Varda Lev-Ram, Paul A. Steinbach, Roger Y. Tsien 2009  Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome.  Science 324: 804-807

 

Supplemental
2009

John Y. Lin, Michael Z. Lin, Paul Steinbach, and Roger Y. Tsien 2009  Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics.  Biophysical Journal 96:1803–1814

 

Supplemental
2008

Stephen R. Adams & Roger Y. Tsien 2008 Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins.  Nature Protocols 3: 1527-1534
2008

Michael Z. Lin, Jeffrey S. Glenn, and Roger Y. Tsien 2008  A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals. Proc. Natl. Acad. Sci. 105: 7744–7749
2008 Nathan C Shaner, Michael Z Lin, Michael R McKeown, Paul A Steinbach, Kristin L Hazelwood, Michael W Davidson & Roger Y Tsien  2008 Improving the photostability of bright monomeric orange and red fluorescent proteins.  Nature Methods 5, 545-551
2008 Samuel Andrew Hires, Yongling Zhu, and Roger Y. Tsien 2008  Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters. Proc. Natl. Acad. Sci. 105: 4411–4416

Supplemental 1
2007 Brent R. Martin, Thomas J. Deerinck, Mark H. Ellisman, Susan S.Taylor, and Roger Y.Tsien - Isoform-Specific PKA Dynamics Revealed by Dye-Triggered Aggregation and DAKAP1α-Mediated Localizationin Living Cells.  Chemistry & Biology 14, 1031–1042

Supplemental 1 (AVI)
Supplemental 2 (AVI)
2007 Oded Tour, Stephen R. Adams, Rex A. Kerr, Rene M. Meijer, Terrence J. Sejnowski, Richard W. Tsien, and Roger Y. Tsien  - Calcium Green FlAsH as a genetically targeted small-molecule calcium indicator.   Nature Chemical Biology. 3: 423-431

Supplemental 1
Supplemental 2
Supplemental 3
Supplemental 4 (AVI)
Supplemental 5 (AVI)
Supplemental 6 (AVI)
Supplemental 7 (AVI)
Supplemental 8
2007 Hui-wang Ai, Nathan C. Shaner,  Zihao Cheng,  Roger Y. Tsien, and Robert E. Campbell 2007 - Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, 5904-5910
2007 Hauser, C.T.,  and Tsien, R.Y. 2007  A hexahistidine-Zn2+-dye label reveals STIM1
surface exposure.  Proc. Natl. Acad. Sci. 104: 3693–3697
2006 Wang, L., and Tsien, R.Y. 2006. Evolving proteins in mammalian cells using somatic hypermutation. Nature Protocols 1: 1346-1350.
2006 Timothy A. Dunn, Chih-Tien Wang, Michael A. Colicos, Manuela Zaccolo, Lisa M. DiPilato, Jin Zhang, Roger Y. Tsien, and Marla B. Feller 2006  Imaging of cAMP levels and protein kinase A activity reveals that retinal waves drive oscillations in second-messenger cascades.  J Neuroscience  26(49): 12807–12815
2006 Guido M. Gaietta, Ben N. G. Giepmans, Thomas J. Deerinck, W. Bryan Smith, Lucy Ngan, Juan Llopis, Stephen R. Adams, Roger Y. Tsien, and Mark H. Ellisman 2006  Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc. Natl. Acad. Sci. 103: 17777–17782
2006 Jeffrey J. Saucerman, JinZhang, Jody C. Martin, Lili X. Peng, Antine E. Stenbit, Roger Y. Tsien, and Andrew D. McCulloch 2006   Systems analysis of PKA-mediated phosphorylation gradients in live cardiac myocytes.  Proc. Natl. Acad. Sci. 103: 12923–12928
2006 Xiaokun Shu, Nathan C. Shaner, Corinne A.Yarbrough, Roger Y. Tsien, and S. James Remington 2006  Novel chromophores and buried charges control color in mFruits.  Biochemistry 45: 9639-9647
2006 Amy E. Palmer, Marta Giacomello, Tanja Kortemme, Andrew Hires, Varda Lev-Ram, David Baker, and Roger Y. Tsien. 2006  Ca2+ Indicators based on computationally redesigned calmodulin-peptide pairs.  Chemistry & Biology 13: 521–530
2006 Jeremy R. Babendure, Jennie L. Babendure, Jian-Hua Ding and Roger Y. Tsien. 2006  Control of mammalian translation by mRNA structure near caps.   RNA,12: 851–861.

Supplemental Material
2005

Brent R. Martin, Ben N.G. Giepmans, Stephen R. Adams & Roger Y. Tsien. 2005 Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity. Nature Biotechnol. 10:1308-1314.

Supplemental Material
2005

Jin Zhang, Christopher J. Hupfeld, Susan S. Taylor, Jerrold M. Olefsky & Roger Y. Tsien. 2005 Insulin disrupts Beta-adrenergic signaling to protein kinase A adipocytes. Nature, 437: 569-573.

Supplement 01

Supplement 02
2005 Hoffmann, C., Gaietta, G., Bünemann, M., Adams, S.R., Oberdorff-Maass, S., Behr, B., Vilardaga, J., Tsien, R.Y., Ellisman, M.H., and Lohse, M.J. 2005 A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells, Nature Methods 2: 171-176
2005 Yingxiao Wang1, Elliot L. Botvinick, Yihua Zhao, Michael W. Berns, Shunichi Usami, Roger Y. Tsien & Shu Chien 2005 Visualizing the mechanical activation of Src, Nature 434: 1040-1045
2005 JC Goldstein, C Munoz-Pinedo, J-E Ricci, SR Adams, A Kelekar, M Schuler, RY Tsien and DR Green 2005  Cytochrome c is released in a single step during apoptosis, Cell Death and Differentiation 12: 453–462
2004 Tao Jiang, Emilia S. Olson, Quyen T. Nguyen, Melinda Roy, Patricia A. Jennings, and Roger Y. Tsien 2004 Tumor imaging by means of proteolytic activation of cell-penetrating peptides. Proc. Natl. Acad. Sci. 101: 17867-17872.  Supplementary Material
2004 Nathan C. Shaner, Robert E. Campbell, Paul Steinbach, Ben N. G. Giepmans, Amy E. Palmer and Roger Y. Tsien. 2004 Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp.red fluorescent protein. Nature Biotech.  22: 1567-1572
2004 Amy E. Palmer, Can Jin, John C. Reed and Roger Y. Tsien 2004 Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor Proc. Natl. Acad. Sci.101: 17404-17409.
2004 Lei Wang, W. Coyt Jackson, Paul A. Steinbach, and Roger Y. Tsien 2004 Evolution of new nonantibody proteins via iterative somatic hypermutation Proc. Natl. Acad. Sci.101: 16745-16749.
2004 Toshiaki Furuta, Hiroko Takeuchi, Masahiro Isozaki, Yasuhiro Takahashi, Makoto Kanehara, Masazumi Sugimoto, Takayoshi Watanabe, Kousei Noguchi, Timothy M. Dore, Takashi Kurahashi, Michiko Iwamura, and Roger Tsien. 2004 Bhc-cNMPs as either Water -Soluble or Membrane-Permeant Photoreleasable Cyclic Nucleotides for both One- and Two-Photon Excitation. Chem. Biochem. 5: 1119-1128.
2004 Colette T. Dooley, Timothy M. Dore, George T. Hanson, W. Coyt Jackson, S. James Remington, and Roger Y. Tsien 2004 Imaging Dynamic Redox Changes in Mammalian Cells with Green Fluorescent Protein Indicators J. Biol. Chem., May 2004; 279: 22284 - 22293
2004 George T. Hanson, Robert Aggeler, Devin Oglesbee, Mark Cannon, Roderick A. Capaldi, Roger Y. Tsien, and S. James Remington 2004 Investigating Mitochondrial Redox Potential with Redox-sensitive Green Fluorescent Protein Indicators J. Biol. Chem., Mar 2004; 279: 13044 -13053.
2003 Ju, W., Morishita, W., Tsui, J., Gaietta, G., Deerink, T.J., Adams, S.R., Garner, C.G., Tsien, R.Y., Ellisman, M.H., and Malenka, R.C. 2004 Activity-dependent regulation of dendritic sysnthesis and trafficking of AMPA receptors. Nat. Neurosci. 7: 244-253
2003 Ray, P., De, A., Min, J-J., Tsien, R.Y. and Gambhir, S.S. 2004 Imaging tri-fusion multimodality reporter gene expression in living subjects. Cancer Res. 64: 1323-1330.
2003 Lev-Ram, V., Mehta, S.B., Kleinfeld, D., and Tsien, R.Y. 2003 Reversing cerebellar long-term depression. Proc. Natl. Acad. Sci. 100: 15989-15993
2003 Hasegawa,S., Jackson, W.C., Tsien, R.Y., and Rao, J-H. 2003 Imaging tetrahymena ribozyme splicing activity in single live mammalian cells Proc. Natl. Acad. Sci. 100: 14892–14896.
2003 Tour,O., Meijer,R.M., Zacharias,D.A., Adams.S.R. and Tsien, R.Y. 2003 Genetically targeted chromophore-assisted light inactivation Nat.Biotech 21: 1505-1508.
2003 Babendure, J.R., Adams,S.R.and Tsien, R.Y. 2003 Aptamers Switch on Fluorescence of Triphenylmethane Dyes. J. Am. Chem. Soc. 125: 14716-14717.
2003 Gao, W., Xing, B., Tsien, R.Y., and Rao, J-H. 2003. Novel fluorogenic substrates for imaging B-Lactamase Gene Expression. J.Am.Chem.Soc. 125: 11146-11147.
2003 Violin, J.D., Zhang, J., Tsien, R.Y., and Newton, A.C. 2003. A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J. Cell Biol. 161: 899-909.
2003 Tsai, P.S., Friedman, B., Ifarraguerri, A.I., Thompson, B.D., Lev-Ram, V., Schaffer, C.B., Xiong, Q., Tsien, R.Y., Squier, J.A., and Kleinfeld, D. 2003. All-optical histology using ultrashort laser pulses. Neuron 39: 27-41
2003 Yu D, Baird GS, Tsien RY, Davis RL. 2003 Detection of calcium transients in Drosophila mushroom body neurons with camgaroo reporters. J Neuroscience  23: 64-72.
2002 Lev-Ram, V., Wong, S.T.,  Storm, D.R., and Tsien, R.Y. 2002 A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP.  Proc. Natl. Acad. Sci. 99: 8389-8393.
2002

Campbell, R.E., Tour, O., Palmer, A.E., Steinbach, P., Baird, G.S., Zacharias, D.A., and Tsien, R.Y. 2002. A monomeric red fluorescent protein. Proc. Natl. Acad. Sci. 99: 7877-7882.
2002

Adams, S.R., Campbell, R.E., Gross, L.A., Martin, B.R., Walkup, G.K., Yao, Y., Llopis, J., and Tsien, R.Y. 2002. New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications. J. Am. Chem. Soc., 124: 6063-6076.
2002 Zacharias,D.A., Violin,J.D., Newton,A.C., Tsien R.Y. 2002 Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells Science 296: 913-916.
2002 Gaietta, G., Deerinck, T.J., Adams, S.R., Bouwer, J., Tour,O., Laird, D.W., Sosinsky, G.E., Tsien, R.Y. and Ellisman, M.H. 2002 Multicolor and Electron Microscopic Imaging of Connexin Trafficking. Science 296: 503-507.
2001

Zhang, J.,  Ma, Y., Taylor, S.S., and Tsien, R.Y. 2001 Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering Proc. Natl. Acad. Sci. 98: 14997-15002.
2001 Ting, A.Y.,  Kain, K.H., Klemke, R.L. and Tsien, R.Y. 2001 Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells Proc.Natl. Acad. Sci 98: 15003-15008.
2001

Wu, M.M., Grabe, M., Adams, S., Tsien, R. Y., Moore, H-P. H., and Machen, T. E. 2001.  Mechanisms of pH regulation in the regulated secretory pathway. J. Biol. Chem. 276: 33027-33035.
2001

Burdette, S.C., Walkup, G.K., Spingler, B., Tsien, R.Y. and Lippard, S.J. 2001. Fluorescent sensors for Zn2+ based on a fluorescein platform: Synthesis, properties and intracellular distribution. J. Am. Chem. Soc. 123: 7831-7841.
2001

Ando, H., Furuta, T., Tsien, R.Y., and Okamoto, H. 2001. Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos. Nature Genetics 28: 317-325.
2001

Chan, F, Siegel, R.M., Zacharias, D., Swofford, R., Holmes, K.L., Tsien, R.Y., Lenardo, M.J. (2001) Fluorescence Resonance Energy Transfer Analysis of Cell Surface Receptor Interactions and Signaling Using Spectral Variants of the Green Fluorescent Protein  Cytometry. 44: 361-368  

2001 Griesbeck,O.; Baird,G.S.; Campbell,R.E.; Zacharias,D.A.; Tsien,R.Y. 2001 Reducing the environmental sensitivity of yellow fluorescent protein. mechanism and applications. J.Biol.Chem. 276: 29188-29194.
2001 Honda, A., Adams, S.R., Sawyer, C.L., Lev-Ram, V., Tsien, R.Y., and Dostmann, W.R.G. 2001. Spatiotemporal dynamics of guanosine 3',5'-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator.  Proc. Natl. Acad. Sci., 98: 2437-2442..
2000 Kapahi, P., Takahashi, T., Natoli, G., Adams, S.R., Chen, Y., Tsien, R.Y. and Karin, M. 2000. Inhibition of NF-κB activation by arsenite through reaction with a critical cysteine in the activation loop of IκB kinase.  J. Biol. Chem. 275: 36062-36066.
2000 Heikal, A.A., Hess, S.T., Baird, G.S., Tsien, R.Y., and Webb, W.W. 2000.  Molecular Spectroscopy and Dynamics of Intrinsically Fluorescent Proteins: Coral Red (DsRed) and Yellow (Citrine). Proc. Natl. Acad. Sci., 97: 11996-12001
2000

Gross, L.A., Baird, G.S., Hoffman, R.C., Baldridge, K.K., and Tsien, R.Y. 2000. The structure of the chromophore within DsRed, a red fluorescent protein from coral.  Proc. Natl. Acad. Sci., 97: 11990-11995.
2000 Baird, G.S., Zacharias, D.A., and Tsien, R.Y. 2000.  Biochemistry, mutagenesis, and oligomerization of dsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci., 97: 11984-11989.
2000

Allen, G.J., Chu, S.P., Schumacher, K., Shimazaki, C.T., Vafeados, D., Kemper, A., Hawke, S.D., Tallman, G., Tsien, R.Y., Harper, J.F., Chory, J., and Schroeder, J.I. 2000.  Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant. Science 289: 2338-2342.

2000 Siegel, R.M., Frederiksen, J.K., Zacharias,D.A., Chan, F.K.-M., Johnson, M., Lynch, D., Tsien, R.Y., and Lenardo, M.J. 2000. Fas preassociation required for apoptosis signaling and dominant inhibition by pathogenic mutations. Science 288: 2354-2357.
2000 Kerr, R., Lev-Ram, V., Baird, G., Vincent, P., Tsien, R.Y., Schafer, W.R. 2000. Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans. Neuron 26: 583-594.
2000 Walkup, G.K., Burdette, S.C., Lippard, S.J., and Tsien, R.Y. 2000. A new cell-permeable fluorescent probe for Zn2+.  J. Amer. Chem. Soc. 122: 5644-5645.
2000 Matsuyama, S., Llopis, J., Deveraux, Q.L., Tsien, R.Y., and Reed, J.C. 2000.  Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis.  Nature Cell Biology 2: 318-325.
2000 Llopis, J., Westin, S., Ricote, M., Wang, J., Cho, C.Y., Kurokawa, R., Rose, D.W., Rosenfeld, M.G., Tsien, R.Y., and Glass, C.K. 2000. Ligand-dependent interactions of coactivators SRC-1 and PBP with nuclear hormone receptors can be imaged in live cells and are required for transcription. Proc. Natl. Acad. Sci. USA 97: 4363-4368.
2000 Wu, M.M., Llopis, J., Adams, S., McCaffery, J.M., Kulomaa, M.S., Machen, T.E., Moore, H.-P.H., and Tsien, R.Y. 2000. Organelle pH studies using targeted avidin and fluorescein-biotin. Chemistry & Biology 7: 197-209.
2000 Zaccolo, M., De Giorgi, F., Cho, C.Y., Feng, L., Knapp, T., Negulescu, P.A., Taylor, S.S., Tsien, R.Y., and Pozzan, T. 2000. A genetically encoded, fluorescent indicator for cAMP in living cells. Nature Cell Biology 2: 25-29.
2000 Thomas, D., Lipp, P., Tovey, S.C., Berridge, M.J., Li, W., Tsien, R.Y., and Bootman, M.D. 2000. Microscopic properties of elementary Ca2+ release sites in non‑excitable cells. Current Biology 10: 8-15.
1999 Baird, G.S., Zacharias, D.A., and Tsien, R.Y. 1999. Circular permutation and receptor insertion into green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96: 11241-11246.
1999 Yao, Y., Ferrer-Montiel, A.V., Montal, M., and Tsien, R.Y. 1999. Activation of store-operated Ca2+ current in Xenopus  oocytes requires SNAP-25 but not a diffusible messenger. Cell 98: 475-485.
1999 Cacciatore, T.W., Brodfuehrer, P.D., Gonzalez, J.E., Jiang, T., Adams, S.R., Tsien, R.Y., Kristan, W.B., and Kleinfeld, D. 1999.  Identification of neural circuits by imaging coherent electrical activity with FRET-based dyes. Neuron 23: 449-459.
1999 Li, C.-J., Heim, R., Lu, P., Pu, Y., Tsien, R.Y., and Chang, D.C. 1999.  Dynamic redistribution of calmodulin in HeLa cells during cell division as revealed by a GFP‑calmodulin fusion protein technique.  J. Cell Sci. 112: 1567‑1577.
1999 Lin, P., Yao, Y., Hofmeister, R., Tsien, R.Y., and Farquhar, M.G. 1999. Overexpression of CALNUC (Nucleobindin) increases agonist and thapsigargin releasable Ca2+ storage in the Golgi. J. Cell Biol. 145: 279-289.
1999 Fan, G.Y., Fujisaki, H., Miyawaki, A., Tsay, R.-K., Tsien, R.Y., and Ellisman, M.H. 1999. Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with yellow cameleons.  Biophys. J. 76: 2412-2420.
1999 Miyawaki, A., Griesbeck, O., Heim, R., and Tsien, R.Y. 1999. Dynamic and quantitative Ca2+ measurements using improved cameleons. Proc. Natl. Acad. Sci. USA 96: 2135-2140.
1999

Furuta, T., Wang, S. S.-H., Dantzker, J.L., Dore, T.M., Bybee, W.J., Callaway, E.M., Denk, W., and Tsien, R.Y. 1999. Brominated 7-hydroxycoumarin-4-ylmethyls: Novel photolabile protecting groups with biologically useful cross-sections for two photon photolysis. Proc. Natl. Acad. Sci. USA 96: 1193-1200.
1999 Brown, E.B., Shear, J.B., Adams, S.R., Tsien, R.Y., Webb, W.W. 1999.  Photolysis of caged calcium in femtoliter volumes using two-photon excitation. Biophys. J. 76: 489-499.
1998 Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W. 1998.  b-Lactamase as a marker for gene expression in live zebrafish embryos. Developmental Biol. 203: 290-294.
1998 Griffin, B.A., Adams, S.R., and Tsien, R.Y. 1998. Specific covalent labeling of recombinant protein molecules inside live cells. Science 281: 269-272.
1998 Llopis, J., McCaffery, J.M., Miyawaki, A., Farquhar, M.G., and Tsien, R.Y. 1998. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95: 6803-6808.
1998 Li, W., Llopis, J., Whitney, M., Zlokarnik, G., and Tsien, R.Y. 1998. Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression. Nature 392: 936-941.
1998 Jiang, T., Sweeney, G., Rudolf, M., Klip, A., Traynor-Kaplan, A., Tsien, R.Y. 1998. Membrane-permeant esters of phosphatidylinositol-3,4,5-trisphosphate. J. Biol. Chem. 273: 11017-11024.
1998 Zlokarnik, G., Negulescu, P.A., Knapp, T.E., Mere, L., Burres, N., Feng, L., Whitney, M., Roemer, K., and Tsien, R.Y. 1998. Quantitation of transcription and clonal selection of single living cells using beta-lactamase as reporter. Science 279: 84-88.
1997 Eckmann, L., Rudolf, M.T., Ptasznik, A., Schultz, C., Jiang, T., Wolfson, N., Tsien,  R., Fierer, J., Shears, S.B., Kagnoff, M.F., Traynor-Kaplan, A.E. 1997. D-myo-inositol 1,4,5,6-tetrakisphosphate produced in human intestinal epithelial cells in response to Salmonella invasion inhibits phosphoinositide 3-kinase signaling pathways. Proc. Natl. Acad. Sci. 94: 14456-14460.
1997 Adams, S.R., Lev-Ram, V., and Tsien, R.Y. 1997.  A new caged Ca2+, azid-1, is far more photosensitive than nitrobenzyl-based chelators. Chemistry & Biology 4: 867-878.
1997 Miyawaki, A., Llopis, J., Heim, R., McCaffery, J.M., Adams, J.A., Ikura, M., and Tsien, R.Y. 1997. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388: 882-887.
1997 Wachter, R.M., King, B.A., Heim, R., Kallio, K., Tsien, R.Y., Boxer, S., and Remington, S.J. 1997. Crystal structure and photodynamic behavior of the blue-emission variant Y66H/Y145F of green fluorescent protein. Biochemistry 36: 9759-9765.
1997 Li, W., Schultz, C., Llopis, J., and Tsien, R.Y. 1997. Membrane-permeant esters of inositol polyphosphates: chemical syntheses and biological applications. Tetrahedron 53: 12017-12040.
1997 Dickson, R.M., Cubitt, A.B., Tsien, R.Y., and Moerner, W.E. 1997. On/off blinking and switching behaviour of single green fluorescent protein molecules. Nature 388: 355
1997 Lev-Ram, V., Jiang, T., Wood, J., Lawrence, D.S., and Tsien, R.Y. 1997. Synergies and coincidence requirements between NO, cGMP, and Ca2+ in the induction of cerebellar long-term depression. Neuron 18: 1025-1038.
1997 Yao, Y. and Tsien, R.Y. 1997. Calcium current activated by depletion of calcium stores in Xenopus oocytes. J. Gen. Physiol. 109: 703-715
1997 Lev-Ram, V., Nebyelul, Z., Ellisman, M.H., Huang, P.L., and Tsien, R.Y. 1997. Absence of cerebellar long-term depression in mice lacking neuronal nitric oxide synthase. Learning & Memory 3: 169-177
1997 Gonzalez, J.E. and Tsien, R.Y. 1997. Improved indicators of cell membrane potential that use fluorescence resonance energy transfer. Chemistry & Biology 4: 269-277.
1997 Brejc, K., Sixma, T.K., Kitts, P.A., Kain, S.R., Tsien, R.Y., Ormö, M., and Remington, S.J. 1997. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. 94: 2306-2311.
1996 Arancio, O., Kiebler, M., Lee, C.J., Lev-Ram, V., Tsien, R.Y., Kandel, E.R., and Hawkins, R.D. 1996. Nitric oxide acts directly in the presynaptic neuron to produce long-term potentiation in cultured hippocampal neurons. Cell  87: 1025-1035.
1996 Hempel, C., Vincent, P., Adams, S.R., Tsien, R.Y., and Selverston, A.I. 1996. Spatio-temporal dynamics of cyclic AMP signals in an intact neural circuit. Nature 384: 166-169.
1996 Scheenen, W.J.J.M., Makings, L.R., Gross, L.R., Pozzan, T., and Tsien, R.Y. 1996. Photodegradation of indo-1 and its effect on apparent Ca2+ concentrations. Chemistry & Biology 3: 765-774.
1996 Ormö, M., Cubitt, A.B., Kallio, K., Gross, L.A., Tsien, R.Y., and Remington, S.J. 1996. Crystal structure of Aequorea victoria green fluorescent protein. Science 273: 1392-1395.
1996 Rizzuto, R., Brini, M., De Georgi, F., Rossi, R., Heim, R., Tsien, R.Y., and Pozzan, T. 1996. Double labelling in vivo of subcellular structures with organelle-targeted GFP mutants. Current Biol. 6: 183-188.
1996 Heim, R., and Tsien, R.Y. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths, and fluorescence resonance energy transfer. Current Biol. 6: 178-182.
1995 Gonzalez, J.E., and Tsien, R.Y. 1995. Voltage sensing by fluorescence resonance energy transfer in single cells. Biophys. J. 69: 1272-1280.
1995 Wen, W., Meinkoth, J.L., Tsien, R.Y., and Taylor, S.S. 1995. Identification of a signal for rapid export of proteins from the nucleus. Cell  82: 463-473.
1995 Lev-Ram, V., Makings, L.R., Keitz, P.F., Kao, J.P.Y., and Tsien, R.Y. 1995. Long-term depression in cerebellar Purkinje neurons results from coincidence of nitric oxide and depolarization-induced Ca2+ transients. Neuron 15: 407-415.
1995 Vajanaphanich, M., Schultz, C., Tsien, R.Y., Traynor-Kaplan, A.E., Pandol, S.J. and Barrett, K.E. 1995. Cross-talk between calcium and cAMP-dependent intracellular signalling pathways: implications for synergistic secretion in T84 colonic epithelial cells and rat pancreatic acinar cells. J. Clin. Invest. 96: 386-393
1995 Heim, R., Cubitt, A.B., and Tsien, R.Y. 1995. Improvement of green fluorescent protein by site-directed mutations. Nature 373: 663-664.
1995 Bacskai, B.J., Wallén, P., Lev-Ram, V., Grillner, S., and Tsien, R.Y. 1995. Activity-related calcium dynamics in lamprey motoneurons as revealed by video-rate confocal microscopy. Neuron 14: 19-28.
1995 Randriamampita, C., and Tsien, R.Y. 1995. Degradation of a calcium influx factor (CIF) can be blocked by phosphatase inhibitors or chelation of Ca2+. J. Biol. Chem. 270: 29-32.
1994 Wen, W., Harootunian, A.T., Adams, S.R., Feramisco, J., Tsien, R.Y., Meinkoth, J.L., and Taylor, S.S. 1994. Heat stable protein kinase inhibitors of cAMP-dependent protein kinase carry a nuclear export signal. J. Biol. Chem. 269: 32214-32220.
1994 Heim, R., Prasher, D.C., and Tsien, R.Y. 1994. Wavelength mutations and post-translational autooxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91: 12501-12504.
1994 Schultz, C., Vajanaphanich, M., Genieser, H.G., Jastorff, B., Barrett, K.E., and Tsien, R.Y. 1994. Membrane-permeant derivatives of cyclic AMP optimized for high potency, prolonged activity, or rapid reversibility. Mol. Pharmacol. 46: 702-708.
1994 Vajanaphanich, M., Schultz, C., Rudolf, M.T., Wasserman, M., Enyedi, P., Craxton, A., Shears, S.B., Tsien, R.Y. Barrett, K.E., and Traynor-Kaplan, A. 1994.  Long-term uncoupling of chloride secretion from intracellular calcium levels by Ins(3,4,5,6)P4. Nature 371: 711-714.
1994 Ranganathan, R., Bacskai, B., Tsien, R.Y., and Zuker, C.S. 1994. Cytosolic calcium transients: spatial localization and role in Drosophila photoreceptor cell function. Neuron 13: 837-848.
1994 Deerinck, T.J., Martone, M.E., Lev Ram, V., Green, D.P.L, Tsien, R.Y., Spector, D.L., Huang, S., and Ellisman, M.H. 1994.  Fluorescence photooxidation with eosin: A method for high resolution immunolocalization and in situ hybridization detection for light and electron microscopy. J. Cell Biol. 126: 901-910.
1994 Civitelli, R., Bacskai, B.J., Mahaut-Smith, M.P., Adams, S.R., Avioli, L.V., and Tsien, R.Y. 1994. Single-cell analysis of cyclic AMP response to parathyroid hormone in osteoblastic cells. J. Bone Miner. Res. 9: 1407-1417.
1994 Gurantz, D., Harootunian, A.T., Tsien, R.Y., Dionne, V.E., and Margiotta, J.F. 1994. VIP modulates neuronal acetylcholine receptor function by a cyclic AMP-dependent mechanism. J. Neurosci. 14: 3540-3547.
1994 Asmis, R., Randriamampita, C., Tsien, R.Y., and Dennis, E.A. 1994. Intracellular Ca2+, inositol 1,4,5-trisphosphate and additional signalling in the platelet-activating factor of prostaglandin E2 formation in P388D1 macrophage-like cells. Biochem. J. 298: 543-551.
1994 Makings, L.R., and Tsien, R.Y. 1994. Caged nitric oxide: stable, organic molecules from which nitric oxide can be photoreleased. J. Biol. Chem. 269: 6282-6285.
1994 Fantozzi, D.A., Harootunian, A.T., Wen, W., Taylor, S.S., Feramisco, J.R., Tsien, R.Y., and Meinkoth, J.L. 1994. The thermostable inhibitor of cAMP-dependent protein kinase enhances the rate of export of the kinase catalytic subunit from the nucleus. J. Biol. Chem. 269: 2676-2686
1993 Harootunian, A.T., Adams, S.R., Wen, W., Meinkoth, J.L., Taylor, S.S., and Tsien, R.Y. 1993. Movement of the free catalytic subunit of cAMP dependent protein kinase into and out of the nucleus can be explained by diffusion. Mol. Biol. Cell. 4: 993-1002.
1993 Randriamampita, C., and Tsien, R.Y. 1993. Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx. Nature 263: 809-814.
1993 Hagiwara, M., Brindle, P., Harootunian, A.T., Armstrong, R., Rivier, J., Vale, W., Tsien, R.Y., and Montminy, M.R. 1993. Coupling of hormonal stimulation and transcription via the cyclic AMP-responsive factor CREB is rate limited by nuclear entry of protein kinase A. Mol. Cell Biol. 13: 4852-4859.
1993 LaMorte, V.J., Harootunian, A.T., Spiegel, A.M., Tsien, R.Y., and Feramisco, J.R. 1993. Mediation of growth factor induced DNA synthesis and calcium mobilization by Gq and Gi2. J. Cell Biol. 121: 91-99.
1993 Bacskai, B.J., Hochner, B., Mahaut-Smith, M., Adams, S.R., Kaang, B.-K., Kandel, E.R., and Tsien, R.Y. 1993. Spatially resolved dynamics of cAMP and protein kinase A subunits in Aplysia sensory neurons. Science 260: 222-226.
1993 Schultz, C., Vajanaphanich, M., Harootunian, A.T., Sammak, P.J., Barrett, K.E., and Tsien, R.Y. 1993. Acetoxymethyl esters of phosphates: enhancement of the permeability and potency of cAMP. J. Biol. Chem. 268: 6316-6322.
1992 Sammak, P.J., Adams, S.R., Harootunian, A.T., Schliwa, M., and Tsien, R.Y. 1992. Intracellular cyclic AMP not Ca2+ determines the direction of vesicle movement in melanophores: direct measurements by fluorescence ratio imaging. J. Cell Biol. 117: 57-72.
1991 Harootunian, A.T., Kao, J.P.Y., Paranjape, S., Adams, S.R., Potter, B.V.L., and Tsien, R.Y. 1991. Cytosolic Ca2+ oscillations in REF52 fibroblasts: Ca2+-stimulated IP3 production or voltage-dependent Ca2+ channels as key positive feedback elements. Cell Calcium 12: 153-164.
1991 Adams, S.R., Harootunian, A.T., Buechler, Y.J., Taylor, S.S., and Tsien, R.Y. 1991. Fluorescence ratio imaging of cyclic AMP in single cells. Nature 349: 694-697.
1991 Harootunian, A.T., Kao, J.P.Y., Paranjape, S., and Tsien, R.Y. 1991. Generation of calcium oscillations in fibroblasts by positive feedback between calcium and IP3. Science 251: 75-78.
1990 Kao, J.P.Y., Alderton, J.M., Tsien, R.Y., and Steinhardt, R.A. 1990. Active involvement of Ca2+ in mitotic progression of Swiss 3T3 fibroblasts. J. Cell Biol. 111: 183-196.
1990 Negulescu, P.A., Harootunian, A., Tsien, R.Y., and Machen, T.E. 1990. Fluorescence measurements of cytosolic free Na concentration, influx and efflux in gastric cells. Cell Regulation 1: 259-268.
1990 Tsien, R.Y., and Harootunian, A.T. 1990. Practical design criteria for a dynamic ratio imaging system. Cell Calcium 11: 93-109.
1989 Harootunian, A.T., Kao, J.P.Y., Eckert, B.K., and Tsien, R.Y. 1989. Fluorescence ratio imaging of cytosolic free Na+ in individual fibroblasts and lymphocytes.  J. Biol. Chem. 264: 19458-19467.
1989 Minta, A., and Tsien, R.Y. 1989. Fluorescent indicators for cytosolic sodium. J. Biol. Chem. 264: 19449-19457.
1989 Adams, S.R., Kao, J.P.Y., and Tsien, R.Y. 1989. Biologically useful chelators that take up Ca2+ upon illumination. J. Amer. Chem. Soc. 111: 7957-7968.
1989 Kawanishi, T., Blank, L.M., Harootunian, A.T., Smith, M.T., and Tsien, R.Y. 1989. Ca2+ oscillations induced by hormonal stimulation of individual fura-2-loaded hepatocytes. J. Biol. Chem. 264: 12859-12866.
1989 Kao, J.P.Y., Harootunian, A.T., and Tsien, R.Y. 1989. Photochemically generated cytosolic calcium pulses and their detection by fluo-3. J. Biol. Chem. 264: 8179-8184.
1989 Minta, A., Kao, J.P.Y., and Tsien, R.Y. 1989. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J. Biol. Chem. 264: 8171-8178.
1989 Havran, W.L., Poenie, M., Tigelaar, R.E., Tsien, R.Y., and Allison, J.P. 1989. Phenotypic and functional analysis of gd T cell receptor-positive murine dendritic epidermal clones. J. Immunology 142: 1422-1428.
1988 Langlet, C., Guimezanes, A., Kaldy, P., Boyer, C., Buferne, M., Poenie, M., Tsien, R., Leo, O., Bluestone, J., Leserman, L., and Schmitt-Verhulst, A.-M. 1988. Role of Ti/CD3, Thy-1, and Ly-6 in cytolytic T-cell activation analyzed with Ti loss variants. Ann. N.Y. Acad. Sci. 532: 33-43.
1985 Grynkiewicz, G.; Poenie, M.; and Tsien, R.Y. (1985) A new generation of CA2+ indicators with greatly improved fluorescence properties*  J.Biol.Chem. 260: 3440-3450
1980 Tsien, R.Y. (1980) New calcium indicators and buffers with high selectivity against magnesium and protons.  Biochemisty  19: 2396-2404